| Description: | The PCSK9(D374T)-LDLR TR-FRET Assay Kit is designed to measure the inhibition of the high affinity PCSK9 mutant (D374T) binding to LDLR in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especia |
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| UniProt | Q8NBP7 |
| Synonym(s): | pcsk9, cholesterol, LDL, kexin |
| Supplied As: | Kit comes in a convenient format with purified ATAD2A, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions. |
| Format: | |
| Instructions for use: | See assay kit data sheet for detailed protocol. |
| Storage / Stability: | At least 6 months from date of receipt when stored as directed. |
| Application(s): | Great for screening small molecular inhibitors for drug discovery and HTS applications. |
| Reference(s): | 1. Chan, J.C. et al. (2009). Proc. Natl. Acad. Sci. USA, 106, 9820-9825. 2. Liang, H., et al. (2012). J. Pharmacol. Exp. Ther. 340 2289-236. |
| Notes: | MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips |
| Scientific Category: | PCSK9(D374T)-LDLR TR-FRET Assay Kit |
| Product Type: | |