Description: The PCSK9(D374T)-LDLR TR-FRET Assay Kit is designed to measure the inhibition of the high affinity PCSK9 mutant (D374T) binding to LDLR in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especia
UniProt Q8NBP7
Synonym(s): pcsk9, cholesterol, LDL, kexin
Supplied As: Kit comes in a convenient format with purified ATAD2A, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt when stored as directed.
Application(s): Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): 1. Chan, J.C. et al. (2009). Proc. Natl. Acad. Sci. USA, 106, 9820-9825. 2. Liang, H., et al. (2012). J. Pharmacol. Exp. Ther. 340 2289-236.
Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
Scientific Category: PCSK9(D374T)-LDLR TR-FRET Assay Kit
Product Type: