- Species:Human
- Host Species:HEK293 cells
- MW:73.8 kDa
|
- Genbank #:NM_174936
- Tag(s):C-terminal His, Avi Tags
- a.a:31–692(end)
|
| Description: |
Human proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as FH3, HCHOLA3, and PC9, GenBank Accession No. NM_174936, a.a. 31-692(end), with C-terminal His and Avi tags, MW=73.8 kDa (calculated), expressed in an HEK293 cell expression system |
| UniProt |
Q8NBP7 |
| Synonym(s): |
Biotinylated PCSK9, FH3, HCHOLA3, PC9, NARC1, Neural Apoptosis Regulated Convertase 1 |
| Assay Conditions: |
50 µL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 µM CaCl2, 0.05% Tween-20, 0.1% BSA, and 0–1500 ng biotin-labeled PCSK9) is added to wells precoated with LDLR Ectodomain. Incubate for 2 hr. Add streptavidin-labeled HRP and incubate 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence. |
| Formulation: |
40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol |
| Format: |
Aqueous buffer solution |
| Storage / Stability: |
>6 months at -80°C. |
| Application(s): |
Useful for studying protein binding and screening small molecules for drug discovery. |
| Reference(s): |
1. Abifadel, M., et al., Nat Genet. 2003 Jun,34(2):154-6.
2. Abifadel, M., et al., Hum Mutat. 2009 Apr;30(4):520-9. |
| Notes: |
PCSK9 is autocleaved to the ~14 kDa prodomain (a.a. 31-152) and the ~60 kDa mature form (a.a. 153-692(end)), which run at higher apparent MW by SDS-PAGE. |
| Warning(s): |
Avoid freeze/thaw cycles |
| Scientific Category: |
PCSK9, C-terminal Avi-His, Biotin-labeled |
| Product Type: |
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